diff --git a/django/conf/__init__.py b/django/conf/__init__.py
index cb70a71791..5156f7dd73 100644
--- a/django/conf/__init__.py
+++ b/django/conf/__init__.py
@@ -227,7 +227,7 @@ class Settings:
         if hasattr(time, "tzset") and self.TIME_ZONE:
             # When we can, attempt to validate the timezone. If we can't find
             # this file, no check happens and it's harmless.
-            zoneinfo_root = Path("/usr/share/zoneinfo")
+            zoneinfo_root = Path("@zoneinfo@")
             zone_info_file = zoneinfo_root.joinpath(*self.TIME_ZONE.split("/"))
             if zoneinfo_root.exists() and not zone_info_file.exists():
                 raise ValueError("Incorrect timezone setting: %s" % self.TIME_ZONE)